Journal: Nature Communications
Article Title: Structural basis of membrane potential coupled vectorial CO₂ hydration by the DAB2 complex in chemolithoautotrophs
doi: 10.1038/s41467-026-72558-7
Figure Lengend Snippet: Time-resolved “CO 2 -minus-N 2 ” ATR FTIR difference spectra for E. coli β-CA (ECCA, black), BSA (red), and Dab2 (blue). a After 25 s in the presence of 10% gaseous CO 2 , bands at 2341 and 2337 cm −1 indicate the presence of dissolved CO 2 or protein-bound CO 2 , respectively. b At lower frequencies, the increasingly more positive bands at 1614, 1360, and 1302 cm −1 revealed HCO 3 – formation. All spectra run from light color ( t = 5 s) to full color ( t = 25 s). c Kinetics of CO 2 hydration with ECCA, BSA, and Dab2 . The arrow marks the switch from 100% N 2 to 90% N 2 and 10% CO 2 (t 0 ). Spectra in ( b ) relate to the time frame between 5 and 25 s highlighted in ( c ). Source data are provided as a Source Data file.
Article Snippet: Chloroform dissolved E. coli polar lipid extract (Avanti Research) was evaporated under a gentle stream of nitrogen to from a thin lipid film.
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